Application
Dot blot analysis of Phospho-FOS-T232 polyclonal antibody (Cat# AP3314a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Product Name | Phospho-FOS-T232 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T232 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:0.388888888889 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G0S7 |
|---|---|
| Alternative Names | FOS G0S7 Proto-oncogene c-Fos Cellular oncogene fos G0/G1 switch regulatory protein 7 |
| Molecular Weight(MW) | 40695 Da |
| Function | Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation |
| Tissue Specificity | This Phospho-FOS-T232 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T232 of human FOS. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2353 |
|---|
Application
Dot blot analysis of Phospho-FOS-T232 polyclonal antibody (Cat# AP3314a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Application Notes | DB:0.388888888889 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
