Application
Western blot analysis of c-fos antibody (C-term) in 293 cell line lysates (35ug/lane). c-fos (arrow) was detected using the purified Pab.Application
c-fos Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | c-fos Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOS; G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G0S7 |
|---|---|
| Alternative Names | FOS G0S7 Proto-oncogene c-Fos Cellular oncogene fos G0/G1 switch regulatory protein 7 |
| Molecular Weight(MW) | 40695 Da |
| Function | Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation |
| Tissue Specificity | This c-fos antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270~299 amino acids from the C-terminal region of human FOS. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2353 |
|---|
Application
Western blot analysis of c-fos antibody (C-term) in 293 cell line lysates (35ug/lane). c-fos (arrow) was detected using the purified Pab.
Application
c-fos Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
