Application
Dot blot analysis of anti-Phospho-HNF4A-pS142 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Confocal immunofluorescent analysis of Phospho-HNF4A-pS142 Antibody(Cat#AP3590a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | Phospho-HNF4A-pS142 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S142 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIF |
| DB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNF4, NR2A1, TCF14 |
|---|---|
| Alternative Names | HNF4A HNF4 NR2A1 TCF14 Hepatocyte nuclear factor 4-alpha Nuclear receptor subfamily 2 group A member 1 Transcription factor 14 Transcription factor HNF-4 |
| Molecular Weight(MW) | 52785 Da |
| Function | Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine |
| Tissue Specificity | This Phospho-HNF4A-pS142 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 125~154 amino acids surrounding S142 of human HNF4A. |
| Cellular Localization | Nucleus. |
Application
Dot blot analysis of anti-Phospho-HNF4A-pS142 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Confocal immunofluorescent analysis of Phospho-HNF4A-pS142 Antibody(Cat#AP3590a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | DB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S142 of human HNF4A. |
