Application
Western blot analysis of HNF4A Antibody (N-term) in 293 cell line lysates (35ug/lane). HNF4A (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of HNF4A Antibody (N-term)(Cat#AP9435a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | HNF4A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNF4, NR2A1, TCF14 |
|---|---|
| Alternative Names | HNF4A HNF4 NR2A1 TCF14 Hepatocyte nuclear factor 4-alpha Nuclear receptor subfamily 2 group A member 1 Transcription factor 14 Transcription factor HNF-4 |
| Molecular Weight(MW) | 52785 Da |
| Function | Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine |
| Tissue Specificity | This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116~145 amino acids from the N-terminal region of human HNF4A. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3172 |
|---|
Application
Western blot analysis of HNF4A Antibody (N-term) in 293 cell line lysates (35ug/lane). HNF4A (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of HNF4A Antibody (N-term)(Cat#AP9435a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
