Application
Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X Antibody (S139) (#AE1016a, Lane 1 and 2) and Phospho-Histone H2A.X-S139 Antibody (#AE1016b, Lane 3 and 4).Application
Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-Histone H2A.X-S139 Antibody (#AE1016b, Red).| Product Name | Phospho-Histone H2A.X-S139 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S139 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:500~1:1000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H2AX |
|---|---|
| Alternative Names | Histone H2A.x H2a/x |
| Molecular Weight(MW) | 15145 Da |
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation |
| Tissue Specificity | The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3014 |
|---|
Application
Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X Antibody (S139) (#AE1016a, Lane 1 and 2) and Phospho-Histone H2A.X-S139 Antibody (#AE1016b, Lane 3 and 4).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-Histone H2A.X-S139 Antibody (#AE1016b, Red).| Application Notes | WB:1:500~1:1000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
