WB
Western blot analysis of Phospho-Histone H2A.X (S139) on different lysates with Mouse anti-Phospho-Histone H2A.X (S139) antibody at 1/5,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: Jurkat treated with 25μM Etoposide for 16 hours cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate Lane 5: C6 (Rat glioma cell) cell lysate Lane 6: C6 treated with 25μM Etoposide for 5 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Mouse anti-Phospho-Histone H2A.X (S139) antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureIHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: colon cancer. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, citrate buffer (pH 6.0), pressure cooker, 2 minutes. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Testis. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, citrate buffer (pH 6.0), pressure cooker, 2 minutes. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/5,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Product Name | Phospho-Histone H2A.X (S139) Recombinant Antibody - Mouse IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX. |
| Modification | p-S139 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:5000 IHC:1:1000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | H2AX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AFX |
| Gene Full Name | H2A.X variant histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Nucleus, Chromosome |
| SwissProt ID | P16104 |
|---|
WB
Western blot analysis of Phospho-Histone H2A.X (S139) on different lysates with Mouse anti-Phospho-Histone H2A.X (S139) antibody at 1/5,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: Jurkat treated with 25μM Etoposide for 16 hours cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate Lane 5: C6 (Rat glioma cell) cell lysate Lane 6: C6 treated with 25μM Etoposide for 5 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Mouse anti-Phospho-Histone H2A.X (S139) antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: colon cancer. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, citrate buffer (pH 6.0), pressure cooker, 2 minutes. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Testis. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, citrate buffer (pH 6.0), pressure cooker, 2 minutes. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/5,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Application Notes | WB:1:5000 IHC:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
