WB
Western blot analysis using H2AFX mouse mAb against HEK293 (1), HepG2 (2), Jurkat (3),Hela (4), Raji (5), K562 (6) and HCT116 (7) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using H2AFX mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using H2AFX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.FC
Flow cytometric analysis of Hela cells using H2AFX mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to H2AFX |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human H2AFX expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCMICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:50-1:200 ICC/IF:1:200-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AFX |
| Gene Full Name | H2A.X variant histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Alternative Names | H2AX H2A.X H2A/X |
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Chromosome,Nucleosome core,Nucleus. |
WB
Western blot analysis using H2AFX mouse mAb against HEK293 (1), HepG2 (2), Jurkat (3),Hela (4), Raji (5), K562 (6) and HCT116 (7) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using H2AFX mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using H2AFX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
FC
Flow cytometric analysis of Hela cells using H2AFX mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:50-1:200 ICC/IF:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
