IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue using HistoneH2AX Rabbit mAb at a dilution of1:4000 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using HistoneH2AX Rabbit mAb at a dilution of1:4000 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using HistoneH2AX Rabbit mAb at a dilution of1:4000 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2AX Rabbit mAbantibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitativePCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Product Name | Histone H2AX Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPIHC |
| IHC:1:1000-1:4000 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AFX |
| Gene Full Name | H2A.X variant histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Alternative Names | H2A.X H2A/X H2AFX Histone H2AX |
| Cellular Localization | Chromosome, Nucleus. |
IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue using HistoneH2AX Rabbit mAb at a dilution of1:4000 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using HistoneH2AX Rabbit mAb at a dilution of1:4000 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using HistoneH2AX Rabbit mAb at a dilution of1:4000 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.
ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2AX Rabbit mAbantibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitativePCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Application Notes | IHC:1:1000-1:4000 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
