WB
Western blot analysis of various lysates using Phospho-Histone H2AX-S139 Rabbit mAb at1:7000 dilution incubated overnight at 4℃. 293T,NIH/3T3 and C6 cells were treated with UV at roomtemperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20 s.IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis tissue usingPhospho-Histone H2AX-S139 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior toIHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen tissue using PhosphoHistone H2AX-S139 Rabbit mAb ata dilution of 1:1000 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate Buffer (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of 293T cells (treated withUV) and 293T cells (untreated) usingPhospho-Histone H2AX-S139 Rabbit mAb( dilution 1:5000) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 100x.ICC/IF
Confocal imaging of NIH/3T3 cells (treatedwith UV) and NIH/3T3 cells (untreated) usingPhospho-Histone H2AX-S139 Rabbit mAb(dilution 1:5000) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 100x.| Product Name | Phospho-Histone H2AX-S139 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | P-S139 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:4000-1:16000 IHC:1:500-1:2000 ICC/IF:1:1000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AFX |
| Gene Full Name | H2A.X variant histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Alternative Names | H2A.X H2A/X H2AFX Phospho-Histone H2AX-S139 |
| Molecular Weight(MW) | 15kDa |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates using Phospho-Histone H2AX-S139 Rabbit mAb at1:7000 dilution incubated overnight at 4℃. 293T,NIH/3T3 and C6 cells were treated with UV at roomtemperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20 s.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis tissue usingPhospho-Histone H2AX-S139 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior toIHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen tissue using PhosphoHistone H2AX-S139 Rabbit mAb ata dilution of 1:1000 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate Buffer (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of 293T cells (treated withUV) and 293T cells (untreated) usingPhospho-Histone H2AX-S139 Rabbit mAb( dilution 1:5000) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 100x.
ICC/IF
Confocal imaging of NIH/3T3 cells (treatedwith UV) and NIH/3T3 cells (untreated) usingPhospho-Histone H2AX-S139 Rabbit mAb(dilution 1:5000) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 100x.| Application Notes | WB:1:4000-1:16000 IHC:1:500-1:2000 ICC/IF:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
