WB
Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.WB
Western blot analysis of Phospho-Histone H2A.X (Ser139) in untreated or Hydroxyureatreated Hela and A549 lysates using Phospho-Histone H2A.X (Ser139) antibody(upper) or betaActin antibody(lower).ICC/IF
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody.ICC/IF
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.| Product Name | Mouse Monoclonal Antibody to Phospho-Histone H2A.X (Ser139) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X. |
| Modification | p-Ser139 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AFX |
| Gene Full Name | H2A.X variant histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Alternative Names | H2A.X H2AFX H2a/x HIST5-2AX Histone H2A.X gamma H2A.X |
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Chromosome,Nucleosome core,Nucleus |
WB
Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.
WB
Western blot analysis of Phospho-Histone H2A.X (Ser139) in untreated or Hydroxyureatreated Hela and A549 lysates using Phospho-Histone H2A.X (Ser139) antibody(upper) or betaActin antibody(lower).
ICC/IF
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody.
ICC/IF
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
