Application
Western analysis of cell extracts from 293 cells transfected with Flag-Bad, using Phospho-mBad-Ser155 Antibody.| Product Name | Phospho-mBad-Ser155 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Ser155 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 27AT381 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Bbc6 |
|---|---|
| Alternative Names | Bad Bbc6 Bcl2 antagonist of cell death Bcl-2-binding component 6 Bcl-xL/Bcl-2-associated death promoter |
| Molecular Weight(MW) | 22080 Da |
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 Appears to act as a link between growth factor receptor signaling and the apoptotic pathways |
| Tissue Specificity | This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic peptide corresponding to sequences in Ser155 region of mouse Bad and residue Ser155 is phosphorylated. |
| Cellular Localization | Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm |
Application
Western analysis of cell extracts from 293 cells transfected with Flag-Bad, using Phospho-mBad-Ser155 Antibody.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
