Application
Dot blot analysis of anti-Phospho-MKK3-S189 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Western blot analysis of anti-Phospho-MKK3 Pab in HL60 cell line lysate (35ug/lane). Phospho-MKK3(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-MKK3-S189 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP2K3; MEK3; MKK3; PRKMK3; SKK2; Dual specificity mitogen-activated protein kinase kinase 3; MAPK/ERK kinase 3; Stress-activated protein kinase kinase 2 |
| Modification | p-S189 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBWBIHC |
| DB:1:500 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK3 MKK3 PRKMK3 SKK2 |
|---|---|
| Alternative Names | MAP2K3 MEK3 MKK3 PRKMK3 SKK2 Dual specificity mitogen-activated protein kinase kinase 3 MAPK/ERK kinase 3 Stress-activated protein kinase kinase 2 |
| Molecular Weight(MW) | 39318 Da |
| Function | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38 |
| Tissue Specificity | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues |
| Entrez Gene | 5606 |
|---|
Application
Dot blot analysis of anti-Phospho-MKK3-S189 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Western blot analysis of anti-Phospho-MKK3 Pab in HL60 cell line lysate (35ug/lane). Phospho-MKK3(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | DB:1:500 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
