Application
The anti-Phospho-p16-INK4A-S140 Pab is used in Western blot to detect Phospho-p16-INK4A-S140 in A2058 tissue lysateApplication
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-p16-INK4A-S140 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN2A; CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1; p16-INK4a |
| Modification | p-S140 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDKN2 MTS1 |
|---|---|
| Alternative Names | CDKN2A CDKN2 MTS1 Cyclin-dependent kinase inhibitor 2A isoforms 1/2/3 Cyclin-dependent kinase 4 inhibitor A Multiple tumor suppressor 1 p16-INK4a |
| Molecular Weight(MW) | 16533 Da |
| Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein |
| Tissue Specificity | Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 1029 |
|---|
Application
The anti-Phospho-p16-INK4A-S140 Pab is used in Western blot to detect Phospho-p16-INK4A-S140 in A2058 tissue lysate
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
