Dot blot analysis of anti-Phospho-PBK-pT9 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
PBK; TOPK; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; T-LAK cell-originated protein kinase
Modification
p-T9
Key Feature
Clonality
Polyclonal
Isotype
Ig
Host Species
Rabbit
Tested Applications
DB
DB:1:500
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Gene Synonyms
TOPK
Alternative Names
PBK TOPK Lymphokine-activated killer T-cell-originated protein kinase Cancer/testis antigen 84 MAPKK-like protein kinase Nori-3 PDZ-binding kinase Spermatogenesis-related protein kinase T-LAK cell-originated protein kinase
Molecular Weight(MW)
36085 Da
Function
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage
Tissue Specificity
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules
Dot blot analysis of anti-Phospho-PBK-pT9 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application Notes
DB:1:500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.