Application
Dot blot analysis of anti-PPM1A-pS Phospho-specific Pab (Cat.AP3436a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-PPM1A-pS375 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPM1A; PPPM1A; Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; Protein phosphatase IA |
| Modification | p-S375 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPPM1A |
|---|---|
| Alternative Names | PPM1A PPPM1A Protein phosphatase 1A Protein phosphatase 2C isoform alpha Protein phosphatase IA |
| Molecular Weight(MW) | 42448 Da |
| Function | Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling |
| Tissue Specificity | This Phospho-PPM1A-pS375 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S375 of human PPM1A. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5494 |
|---|
Application
Dot blot analysis of anti-PPM1A-pS Phospho-specific Pab (Cat.AP3436a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
