Application
A. Western blot analysis on SH-SY5Y cell lysate using anti-PP2C-alpha RabMAb (Cat. #AJ1646a), 1:2000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-PP2C-alpha RabMAb (Cat. #AJ1646a).Application
C. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PP2C-alpha RabMAb (Cat. #AJ1646a).| Product Name | PP2C alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPM1A, PPPM1A, Protein phosphatase 1A, Protein phosphatase 2C isoform alpha |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1684Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPPM1A |
|---|---|
| Alternative Names | PPM1A PPPM1A Protein phosphatase 1A Protein phosphatase 2C isoform alpha |
| Molecular Weight(MW) | 42448 Da |
| Function | Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-terminal of human PP2C alpha protein was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5494 |
|---|
Application
A. Western blot analysis on SH-SY5Y cell lysate using anti-PP2C-alpha RabMAb (Cat. #AJ1646a), 1:2000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-PP2C-alpha RabMAb (Cat. #AJ1646a).
Application
C. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PP2C-alpha RabMAb (Cat. #AJ1646a).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
