Application
Western blot analysis of hPPM1A-C72 (RB06012) in HL-60 cell line lysates (35ug/lane).PPM1A (arrow) was detected using the purified Pab.Application
Western blot analysis of PPM1A (arrow) using rabbit polyclonal hPPM1A-C72 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1A gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PPM1A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPM1A; PPPM1A; Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; Protein phosphatase IA |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPPM1A |
|---|---|
| Alternative Names | PPM1A PPPM1A Protein phosphatase 1A Protein phosphatase 2C isoform alpha Protein phosphatase IA |
| Molecular Weight(MW) | 42448 Da |
| Function | Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling |
| Tissue Specificity | This PPM1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64~94 amino acids from the center region of human PPM1A. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5494 |
|---|
Application
Western blot analysis of hPPM1A-C72 (RB06012) in HL-60 cell line lysates (35ug/lane).PPM1A (arrow) was detected using the purified Pab.
Application
Western blot analysis of PPM1A (arrow) using rabbit polyclonal hPPM1A-C72 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1A gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
