Application
Dot blot analysis of anti-Phospho-hSTAT5a-pS726 Phospho-specific Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Western blot analysis of anti-Phospho-STAT5a-S726 Antibody in CEM cell line lysate (35ug/lane). Phospho-STAT5a(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-STAT5a-S726 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT5A; STAT5; Signal transducer and activator of transcription 5A |
| Modification | p-S726 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBWBIHC |
| DB:1:500 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | STAT5 |
|---|---|
| Alternative Names | STAT5A STAT5 Signal transducer and activator of transcription 5A |
| Molecular Weight(MW) | 90647 Da |
| Function | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation |
| Tissue Specificity | This Phospho-STAT5a-S726 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S726 of human STAT5a. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation |
| Entrez Gene | 6776 |
|---|
Application
Dot blot analysis of anti-Phospho-hSTAT5a-pS726 Phospho-specific Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Western blot analysis of anti-Phospho-STAT5a-S726 Antibody in CEM cell line lysate (35ug/lane). Phospho-STAT5a(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | DB:1:500 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
