Application
A. Western blot analysis on A431 cell lysate using anti- Phospho-Stat5 (Y694) RabMAb (Cat. #AJ1741b). Cell lysates were (1) untreated, (2) treated with EGF, or (3) treated with Phosphatase.Application
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Phospho-Stat5 (Y694) RabMAb (Cat. #AJ1741b).| Product Name | Stat-5 Antibody Phospho (pY694) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT5A, STAT5, Signal transducer and activator of transcription 5A |
| Modification | p-Y694 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E208 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | STAT5 |
|---|---|
| Alternative Names | STAT5A STAT5 Signal transducer and activator of transcription 5A |
| Molecular Weight(MW) | 90647 Da |
| Function | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human Stat5 was used as immunogen. The antibody only detects Stat5 phosphorylated on Tyrosine 694. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation |
| Entrez Gene | 6776 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti- Phospho-Stat5 (Y694) RabMAb (Cat. #AJ1741b). Cell lysates were (1) untreated, (2) treated with EGF, or (3) treated with Phosphatase.
Application
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Phospho-Stat5 (Y694) RabMAb (Cat. #AJ1741b).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
