Dot blot analysis of anti-Phospho-TWF7-pY327 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
TWF1; PTK9; Twinfilin-1; Protein A6; Protein tyrosine kinase 9
Modification
p-Y327
Key Feature
Clonality
Polyclonal
Isotype
Ig
Host Species
Rabbit
Tested Applications
DB
DB:1:500
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Gene Synonyms
PTK9
Alternative Names
TWF1 PTK9 Twinfilin-1 Protein A6 Protein tyrosine kinase 9
Molecular Weight(MW)
40283 Da
Function
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity)
Tissue Specificity
Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver
Cellular Localization
Cytoplasm. Cytoplasm, cytoskeleton (By similarity). Note=Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts (By similarity)
Dot blot analysis of anti-Phospho-TWF7-pY327 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application Notes
DB:1:500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.