Application
Western blot analysis of Phospho-TWF1-pY321.ctrl in mouse bladder tissue lysates (35ug/lane). TWF1 (arrow) was detected using the purified Pab.Application
Western blot analysis of TWF1 (arrow) using rabbit polyclonal TWF1 Antibody (Y321) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TWF1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with TWF1 Antibody (Y321) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PTK9 (TWF1) Antibody (Y321) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTK9 |
|---|---|
| Alternative Names | TWF1 PTK9 Twinfilin-1 Protein A6 Protein tyrosine kinase 9 |
| Molecular Weight(MW) | 40283 Da |
| Function | Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity) |
| Tissue Specificity | Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton (By similarity). Note=Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts (By similarity) |
Application
Western blot analysis of Phospho-TWF1-pY321.ctrl in mouse bladder tissue lysates (35ug/lane). TWF1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of TWF1 (arrow) using rabbit polyclonal TWF1 Antibody (Y321) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TWF1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with TWF1 Antibody (Y321) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
