Application
Western blot analysis of PINK (arrow) using mouse monoclonal PINK antibody (Cat.#AM6406b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PINK gene (Lane 2) (Origene Technologies)Application
PINK1 Monoclonal Antibody (Cat. #AM6406b) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINK1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PINK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PINK1; Serine/threonine-protein kinase PINK1, mitochondrial; BRPK; PTEN-induced putative kinase protein 1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 38CT20.8.5 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PINK1 Serine/threonine-protein kinase PINK1 mitochondrial BRPK PTEN-induced putative kinase protein 1 |
|---|---|
| Molecular Weight(MW) | 62769 Da |
| Function | Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation |
| Tissue Specificity | Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm, cytosol |
| Entrez Gene | 65018 |
|---|
Application
Western blot analysis of PINK (arrow) using mouse monoclonal PINK antibody (Cat.#AM6406b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PINK gene (Lane 2) (Origene Technologies)
Application
PINK1 Monoclonal Antibody (Cat. #AM6406b) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINK1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
