Application
A. Immunohistochemical analysis of untreated paraffin-embedded lymph node metastatic tissue using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).Application
B. Immunohistochemical analysis of Phosphatase-treated paraffin-embedded lymph node metastatic tissue using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).Application
C. Western blot analysis on K562 cell lysate using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b), 1:2,500 dilution. Cell lysates were either (1) untreated or (2) treated with Alkaline Phosphatase.Application
D. Immunofluorescent analysis of untreated HeLa cells using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).Application
E. Immunofluorescent analysis of Alkaline Phosphatase-treated HeLa cells using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).| Product Name | PKA, RII Antibody Phospho (pS99) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S99 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E151 |
| Host Species | Rabbit |
| Tested Applications | IHCWBIF |
| IHC:1:250 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PKR2 PRKAR2 |
|---|---|
| Molecular Weight(MW) | 45518 Da |
| Function | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase |
| Tissue Specificity | Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and the cell membrane |
| Entrez Gene | 5576 |
|---|
Application
A. Immunohistochemical analysis of untreated paraffin-embedded lymph node metastatic tissue using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).
Application
B. Immunohistochemical analysis of Phosphatase-treated paraffin-embedded lymph node metastatic tissue using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).
Application
C. Western blot analysis on K562 cell lysate using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b), 1:2,500 dilution. Cell lysates were either (1) untreated or (2) treated with Alkaline Phosphatase.
Application
D. Immunofluorescent analysis of untreated HeLa cells using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).
Application
E. Immunofluorescent analysis of Alkaline Phosphatase-treated HeLa cells using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).| Application Notes | IHC:1:250 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
