Application
A. Western blot analysis on K562 cell lysate using anti- PKA RII (C-term) RabMAb (Cat. #AJ1614a), dilution 50,000Application
B. Immunohistochemical analysis of paraffin-embedded human normal breast tissue using anti-PKA RII (C-term) RabMAb (Cat. #AJ1614a).Application
C. Immunofluorescent staining of HeLa cells using anti-PKA RII (C-term) RabMAb (Cat. #AJ1614a).| Product Name | PKA, RII Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAR2A, PKR2, PRKAR2, cAMP-dependent protein kinase type II-alpha regulatory subunit |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y116 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PKR2 PRKAR2 |
|---|---|
| Alternative Names | PRKAR2A PKR2 PRKAR2 cAMP-dependent protein kinase type II-alpha regulatory subunit |
| Molecular Weight(MW) | 45518 Da |
| Function | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase |
| Tissue Specificity | Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and the cell membrane |
| Entrez Gene | 5576 |
|---|
Application
A. Western blot analysis on K562 cell lysate using anti- PKA RII (C-term) RabMAb (Cat. #AJ1614a), dilution 50,000
Application
B. Immunohistochemical analysis of paraffin-embedded human normal breast tissue using anti-PKA RII (C-term) RabMAb (Cat. #AJ1614a).
Application
C. Immunofluorescent staining of HeLa cells using anti-PKA RII (C-term) RabMAb (Cat. #AJ1614a).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
