Application
Formalin-fixed and paraffin-embedded human brain with PO4F3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of PO4F3 Antibody (C-term) in HL-60, MDA-MB435, CEM cell line and mouse brain,cerebellum tissue lysates (35ug/lane).PO4F3 (arrow) was detected using the purified Pab.Application
Fluorescent confocal image of U251 cell stained with PO4F3 Antibody (C-term)(Cat#AP9352b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PO4F3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). PO4F3 immunoreactivity is localized to Nucleus significantly.| Product Name | PO4F3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POU4F3; BRN3C; POU domain, class 4, transcription factor 3; Brain-specific homeobox/POU domain protein 3C |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBIF |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRN3C |
|---|---|
| Alternative Names | POU4F3 BRN3C POU domain class 4 transcription factor 3 Brain-specific homeobox/POU domain protein 3C |
| Molecular Weight(MW) | 37052 Da |
| Function | May play a role in determining or maintaining the identities of a small subset of visual system neurons |
| Tissue Specificity | Brain. Seems to be specific to the retina. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5459 |
|---|
Application
Formalin-fixed and paraffin-embedded human brain with PO4F3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of PO4F3 Antibody (C-term) in HL-60, MDA-MB435, CEM cell line and mouse brain,cerebellum tissue lysates (35ug/lane).PO4F3 (arrow) was detected using the purified Pab.
Application
Fluorescent confocal image of U251 cell stained with PO4F3 Antibody (C-term)(Cat#AP9352b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PO4F3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). PO4F3 immunoreactivity is localized to Nucleus significantly.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
