Application
Immunofluorescence of monoclonal antibody to POLK (AT3371a) on HeLa cell . [antibody concentration 10 ug/ml]Application
Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # AT3371a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Product Name | POLK Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DINB1, DINP, POLQpolymerase (DNA directed) kappa |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6F2 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DINB1 DINP POLQpolymerase (DNA directed) kappa |
|---|---|
| Molecular Weight(MW) | 98809 Da |
| Tissue Specificity | POLK (AAH50718, 1 a.a. ~ 473 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Entrez Gene | 51426 |
|---|
Application
Immunofluorescence of monoclonal antibody to POLK (AT3371a) on HeLa cell . [antibody concentration 10 ug/ml]
Application
Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # AT3371a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
