Application
Figure 1: Western blot analysis using anti-OCT4 monoclonal antibody against PMA treated HepG2 cell lysate (1).| Product Name | POU5F1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OCT3; POU5F1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3A5 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OCT3 OCT4 OTF3 |
|---|---|
| Alternative Names | OCT3 POU5F1 |
| Molecular Weight(MW) | 38571 Da |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency |
| Tissue Specificity | Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues |
| Cellular Localization | Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern |
| Entrez Gene | 5460 |
|---|---|
| Nucleotide Accession | NP_976034.3 |
Application
Figure 1: Western blot analysis using anti-OCT4 monoclonal antibody against PMA treated HepG2 cell lysate (1).| Application Notes | WB:1:500 - 2,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of OCT4 expressed in E. Coli. |
Data sheet for OM218371