Application
Western blot analysis of anti-PPP4C Pab in Hela cell lysate.PPP4C(arrow) was detected using the purified Pab.Application
Western blot analysis of PPP4C(arrow) using rabbit polyclonal PPP4C Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPP4C gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PPP4C Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP4C; PPP4; PPX; Serine/threonine-protein phosphatase 4 catalytic subunit; Protein phosphatase X |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPP4 PPX |
|---|---|
| Alternative Names | PPP4C PPP4 PPX Serine/threonine-protein phosphatase 4 catalytic subunit Protein phosphatase X |
| Molecular Weight(MW) | 35080 Da |
| Function | Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C- PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin |
| Tissue Specificity | This PPP4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human PPP4C. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome |
| Entrez Gene | 5531 |
|---|
Application
Western blot analysis of anti-PPP4C Pab in Hela cell lysate.PPP4C(arrow) was detected using the purified Pab.
Application
Western blot analysis of PPP4C(arrow) using rabbit polyclonal PPP4C Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPP4C gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
