Application
PPP4C Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPP4C antibody detected the PPP4C protein (arrow).Application
PPP4C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPP4C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PPP4C Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP4C; PPP4; PPX; Serine/threonine-protein phosphatase 4 catalytic subunit; Protein phosphatase X |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPP4 PPX |
|---|---|
| Alternative Names | PPP4C PPP4 PPX Serine/threonine-protein phosphatase 4 catalytic subunit Protein phosphatase X |
| Molecular Weight(MW) | 35080 Da |
| Function | Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C- PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin |
| Tissue Specificity | This PPP4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from the Central region of human PPP4C. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome |
| Entrez Gene | 5531 |
|---|---|
| Nucleotide Accession | NP_002711.1 |
Application
PPP4C Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPP4C antibody detected the PPP4C protein (arrow).
Application
PPP4C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPP4C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
