Application
A) Rat B50 brain tumor cell extracts were immunoprecipitated with RIZ1 antibody (cat# AP1202b) or preimmune (ref.9). B)Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis (ref.10).| Product Name | PRDM2 (RIZ1) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRDM2; KMT8; RIZ; PR domain zinc finger protein 2; GATA-3-binding protein G3B; Lysine N-methyltransferase 8; MTB-ZF; MTE-binding protein; PR domain-containing protein 2; Retinoblastoma protein-interacting zinc finger protein; Zinc finger protein RIZ |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT8 RIZ |
|---|---|
| Alternative Names | PRDM2 KMT8 RIZ PR domain zinc finger protein 2 GATA-3-binding protein G3B Lysine N-methyltransferase 8 MTB-ZF MTE-binding protein PR domain-containing protein 2 Retinoblastoma protein-interacting zinc finger protein Zinc finger protein RIZ |
| Molecular Weight(MW) | 188915 Da |
| Function | S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene |
| Tissue Specificity | Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen Isoform 1 is expressed in testis at much higher level than isoform 3 |
| Cellular Localization | Nucleus. |
Application
A) Rat B50 brain tumor cell extracts were immunoprecipitated with RIZ1 antibody (cat# AP1202b) or preimmune (ref.9). B)Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis (ref.10).| Application Notes | IHC:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
