Application
Western blot analysis of anti-PRDM2 Mab (Cat. #AM1194a) in lysate from transformed cells.Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.Application
(A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibodies against RIZ1. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.| Product Name | PRDM2 (RIZ1) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRDM2; KMT8; RIZ; PR domain zinc finger protein 2; GATA-3-binding protein G3B; Lysine N-methyltransferase 8; MTB-ZF; MTE-binding protein; PR domain-containing protein 2; Retinoblastoma protein-interacting zinc finger protein; Zinc finger protein RIZ |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 33AT1045 |
| Host Species | Mouse |
| Tested Applications | WBIP |
| WB:1:1000 IP |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT8 RIZ |
|---|---|
| Alternative Names | PRDM2 KMT8 RIZ PR domain zinc finger protein 2 GATA-3-binding protein G3B Lysine N-methyltransferase 8 MTB-ZF MTE-binding protein PR domain-containing protein 2 Retinoblastoma protein-interacting zinc finger protein Zinc finger protein RIZ |
| Molecular Weight(MW) | 188915 Da |
| Function | S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene |
| Tissue Specificity | Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen Isoform 1 is expressed in testis at much higher level than isoform 3 |
| Cellular Localization | Nucleus. |
| Entrez Gene | 7799 |
|---|
Application
Western blot analysis of anti-PRDM2 Mab (Cat. #AM1194a) in lysate from transformed cells.Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.
Application
(A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibodies against RIZ1. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.| Application Notes | WB:1:1000 IP |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
