Application
A. Western blot analysis on Jurkat cell lysates using anti-phospho Presenilin 1 (pS317) RabMAb (Cat. #AJ1650c), dilution 1:5000. Cells were either (A) untreated (B) treated with TPA.| Product Name | Presenilin-1 Antibody Phospho (pS310) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSEN1, AD3, PS1, PSNL1, Presenilin-1 CTF12, Protein S182 |
| Modification | p-S310 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2001Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AD3 PS1 PSNL1 |
|---|---|
| Alternative Names | PSEN1 AD3 PS1 PSNL1 Presenilin-1 CTF12 Protein S182 |
| Molecular Weight(MW) | 52668 Da |
| Function | Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis |
| Tissue Specificity | Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell surface. Note=Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils |
| Entrez Gene | 5663 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-phospho Presenilin 1 (pS317) RabMAb (Cat. #AJ1650c), dilution 1:5000. Cells were either (A) untreated (B) treated with TPA.| Application Notes | WB:1:5000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
