Application
A. Western blot analysis on fetal brain lysate using anti-PrP RabMAb (Cat. #AJ1652a), dilution 1:10,000.Application
B. Immunohistochemical analysis of brain glioma using anti-PrP RabMAb (Cat. #AJ1652a).| Product Name | Prion Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRNP, PRIP, PRP, Major prion protein, PrP27-30;PrP33-35C;ASCR |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1802Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PRIP PRP |
|---|---|
| Alternative Names | PRNP PRIP PRP Major prion protein PrP27-30 PrP33-35C ASCR |
| Molecular Weight(MW) | 27661 Da |
| Function | May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-terminus of human Prp was used as an immunogen. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Note=Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU(2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis |
| Entrez Gene | 5621 |
|---|
Application
A. Western blot analysis on fetal brain lysate using anti-PrP RabMAb (Cat. #AJ1652a), dilution 1:10,000.
Application
B. Immunohistochemical analysis of brain glioma using anti-PrP RabMAb (Cat. #AJ1652a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
