Application
Western blot analysis of anti-PRNP Antibody (Center) in Ramos cell line lysates (35ug/lane). PRNP(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue with PRNP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of PRNP Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | PRNP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRNP; PRIP; PRP; Major prion protein; ASCR; PrP27-30; PrP33-35C; CD_antigen=CD230; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PRIP PRP |
|---|---|
| Alternative Names | PRNP PRIP PRP Major prion protein ASCR PrP27-30 PrP33-35C CD_antigen=CD230 Flags: Precursor |
| Molecular Weight(MW) | 27661 Da |
| Function | May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity) |
| Tissue Specificity | This PRNP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135~165 amino acids from the Center region of human PRNP. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Note=Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU(2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis |
| Entrez Gene | 5621 |
|---|
Application
Western blot analysis of anti-PRNP Antibody (Center) in Ramos cell line lysates (35ug/lane). PRNP(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue with PRNP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of PRNP Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
