Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKAR2A Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of PRKAR2A Antibody (N-term) in Jurkat cell line lysates (35ug/lane). PRKAR2A (arrow) was detected using the purified Pab.Application
PRKAR2A Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PRKAR2A (arrow) using rabbit polyclonal PRKAR2A Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAR2A gene.| Product Name | PRKAR2A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBFC |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PKR2 PRKAR2 |
|---|---|
| Alternative Names | PRKAR2A PKR2 PRKAR2 cAMP-dependent protein kinase type II-alpha regulatory subunit |
| Molecular Weight(MW) | 45518 Da |
| Function | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase |
| Tissue Specificity | Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and the cell membrane |
| Entrez Gene | 5576 |
|---|
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKAR2A Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of PRKAR2A Antibody (N-term) in Jurkat cell line lysates (35ug/lane). PRKAR2A (arrow) was detected using the purified Pab.
Application
PRKAR2A Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PRKAR2A (arrow) using rabbit polyclonal PRKAR2A Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAR2A gene.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
