Application
A. Western blot analysis on T47D cell lysates using anti-Phospho-PR (pS190) RabMAb (Cat. #AJ1655b), 1:10,000 dilution. Cells were either (A) untreated (B) treated with progesterone.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-PR RabMAb. (Cat. #AJ1655b).| Product Name | Progesterone Antibody Phospho (pS190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PGR, NR3C3, Progesterone receptor, Nuclear receptor subfamily 3 group C member 3 |
| Modification | p-S190 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1516Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NR3C3 |
|---|---|
| Alternative Names | PGR NR3C3 Progesterone receptor Nuclear receptor subfamily 3 group C member 3 |
| Molecular Weight(MW) | 98981 Da |
| Function | The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding serine 190 of human PR was used as an immunogen. This antibody detects PR phophorylated at serine 190. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases |
| Entrez Gene | 5241 |
|---|
Application
A. Western blot analysis on T47D cell lysates using anti-Phospho-PR (pS190) RabMAb (Cat. #AJ1655b), 1:10,000 dilution. Cells were either (A) untreated (B) treated with progesterone.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-PR RabMAb. (Cat. #AJ1655b).| Application Notes | WB:1:2000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
