Application
Western blot analysis of PTPN6 Antibody (Center) in HL-60 cell line lysates (35ug/lane).PTPN6 (arrow) was detected using the purified Pab.Application
Western blot analysis of PTPN6 (arrow) using rabbit polyclonal PTPN6 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2) (Origene Technologies).(2ug/ml)Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PTPN6 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HCP, PTP1C |
|---|---|
| Alternative Names | PTPN6 HCP PTP1C Tyrosine-protein phosphatase non-receptor type 6 Hematopoietic cell protein-tyrosine phosphatase Protein-tyrosine phosphatase 1C Protein-tyrosine phosphatase SHP-1 SH-PTP1 |
| Molecular Weight(MW) | 67561 Da |
| Function | Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis |
| Tissue Specificity | Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells |
| Cellular Localization | Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1 |
| Entrez Gene | 5777 |
|---|
Application
Western blot analysis of PTPN6 Antibody (Center) in HL-60 cell line lysates (35ug/lane).PTPN6 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PTPN6 (arrow) using rabbit polyclonal PTPN6 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2) (Origene Technologies).(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
