Application
Western blot analysis of PUM2 (arrow) using PUM2 Antibody (S182) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PUM2 Antibody (S182) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0235, PUMH2 |
|---|---|
| Alternative Names | PUM2 KIAA0235 PUMH2 Pumilio homolog 2 |
| Molecular Weight(MW) | 114216 Da |
| Function | Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts |
| Tissue Specificity | Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen Expressed in all fetal tissues tested |
| Cellular Localization | Cytoplasm (Probable). Cytoplasmic granule (By similarity). Cytoplasm, perinuclear region. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules (By similarity). Co-localizes with NANOS1 and SNAPIN in the perinuclear region of germ cells |
| Entrez Gene | 23369 |
|---|
Application
Western blot analysis of PUM2 (arrow) using PUM2 Antibody (S182) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
