Application
Western blot analysis on (A) HeLa and (B) 293T cell lysates using anti-Pumilio-2 RabMAb (Cat. #AJ1668a).| Product Name | Pumilio-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3813 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0235, PUMH2 |
|---|---|
| Alternative Names | PUM1 KIAA0099 PUMH1 Pumilio homolog 1 |
| Molecular Weight(MW) | 114216 Da |
| Function | Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts |
| Tissue Specificity | Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen Expressed in all fetal tissues tested |
| Cellular Localization | Cytoplasm (Probable). Cytoplasmic granule (By similarity). Cytoplasm, perinuclear region. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules (By similarity). Co-localizes with NANOS1 and SNAPIN in the perinuclear region of germ cells |
| Entrez Gene | 23369 |
|---|
Application
Western blot analysis on (A) HeLa and (B) 293T cell lysates using anti-Pumilio-2 RabMAb (Cat. #AJ1668a).| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
