Application
PUM2-S136 Antibody western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PUM2 antibody detected the PUM2 protein (arrow).Application
PUM2-S136 Antibody flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PUM2-S136 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0235, PUMH2 |
|---|---|
| Alternative Names | PUM2 KIAA0235 PUMH2 Pumilio homolog 2 |
| Molecular Weight(MW) | 114216 Da |
| Function | Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts |
| Tissue Specificity | Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen Expressed in all fetal tissues tested |
| Cellular Localization | Cytoplasm (Probable). Cytoplasmic granule (By similarity). Cytoplasm, perinuclear region. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules (By similarity). Co-localizes with NANOS1 and SNAPIN in the perinuclear region of germ cells |
| Entrez Gene | 23369 |
|---|---|
| Nucleotide Accession | NP_056132.1 |
Application
PUM2-S136 Antibody western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PUM2 antibody detected the PUM2 protein (arrow).
Application
PUM2-S136 Antibody flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
