Application
PYHIN1 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PYHIN1 antibody detected the PYHIN1 protein (arrow).Application
PYHIN1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYHIN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PYHIN1 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PYHIN1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PYHIN1; IFIX; Pyrin and HIN domain-containing protein 1; Interferon-inducible protein X |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IFIX |
|---|---|
| Alternative Names | PYHIN1 IFIX Pyrin and HIN domain-containing protein 1 Interferon-inducible protein X |
| Molecular Weight(MW) | 55065 Da |
| Function | Major mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells |
| Tissue Specificity | Expressed in spleen, lymph node and peripheral blood leukocytes, and at lower levels in thymus, bone marrow and fetal liver. Down-regulated in breast tumors |
| Cellular Localization | Isoform 1: Nucleus, nucleoplasm. Isoform 5: Nucleus. Nucleus speckle. |
| Entrez Gene | 149628 |
|---|---|
| Nucleotide Accession | NP_945146.1 NP_945148.1 |
Application
PYHIN1 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PYHIN1 antibody detected the PYHIN1 protein (arrow).
Application
PYHIN1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYHIN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PYHIN1 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
