Application
Western blot analysis of hPKM2-N491 (Cat.# AP7173a) in A2058 cell line(lane 1) and mouse brain tissue(lane 2) lysates (35ug/lane). PKM2 (arrow) was detected using the purified Pab.| Product Name | Pyruvate Kinase (PKM2) Antibody (C-term N491) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OIP3, PK2, PK3, PKM2 |
|---|---|
| Alternative Names | PKM OIP3 PK2 PK3 PKM2 Pyruvate kinase isozymes M1/M2 Cytosolic thyroid hormone-binding protein Opa-interacting protein 3 Pyruvate kinase 2/3 Pyruvate kinase muscle isozyme Thyroid hormone-binding protein 1 Tumor M2-PK p58 |
| Molecular Weight(MW) | 57937 Da |
| Function | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival |
| Tissue Specificity | Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity |
| Entrez Gene | 5315 |
|---|
Application
Western blot analysis of hPKM2-N491 (Cat.# AP7173a) in A2058 cell line(lane 1) and mouse brain tissue(lane 2) lysates (35ug/lane). PKM2 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
