Application
Figure 1: Western blot analysis using anti-R-spondin1 monoclonal antibody against recombinant R-spondin1 protein(1) and R-spondin1(aa21-263)-hIgGFc transfected HEK293 cell lysate(2).| Product Name | R-spondin1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RSPO1;CRISTIN3;FLJ40906 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7A6 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RSPO1 CRISTIN3 FLJ40906 |
|---|---|
| Molecular Weight(MW) | 28959 Da |
| Function | Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination |
| Tissue Specificity | Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen |
| Cellular Localization | Secreted (By similarity). |
| Entrez Gene | 284654 |
|---|---|
| Nucleotide Accession | NP_001033722.1 |
Application
Figure 1: Western blot analysis using anti-R-spondin1 monoclonal antibody against recombinant R-spondin1 protein(1) and R-spondin1(aa21-263)-hIgGFc transfected HEK293 cell lysate(2).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of RSPO1 expressed in E. Coli. |
