Application
RAB8A Antibody (Cat. #AM1924b) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the RAB8A antibody detected the RAB8A protein (arrow).| Product Name | RAB8A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB8A; MEL; RAB8; Ras-related protein Rab-8A; Oncogene c-mel |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 261CT1.3.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEL RAB8 |
|---|---|
| Alternative Names | RAB8A MEL RAB8 Ras-related protein Rab-8A Oncogene c-mel |
| Molecular Weight(MW) | 23668 Da |
| Function | May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization |
| Tissue Specificity | This RAB8A monoclonal antibody is generated from mouse immunized with RAB8A recombinant protein. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Golgi apparatus. Cytoplasm, perinuclear region Cell projection. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side (By similarity). Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP- bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP- bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Recruited to phagosomes containing S.aureus or M.tuberculosis |
| Entrez Gene | 4218 |
|---|---|
| Nucleotide Accession | NP_005361.2 |
Application
RAB8A Antibody (Cat. #AM1924b) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the RAB8A antibody detected the RAB8A protein (arrow).| Application Notes | WB:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
