Application
Immunofluorescence of monoclonal antibody to RAD18 (AT3545a) on HeLa cell . [antibody concentration 25 ug/ml]Application
Western blot analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody (M01), clone 3H7 (Cat # AT3545a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Product Name | RAD18 Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF73RAD18 homolog (S. cerevisiae) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 3H7 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RNF73RAD18 homolog (S. cerevisiae) |
|---|---|
| Molecular Weight(MW) | 56223 Da |
| Tissue Specificity | RAD18 (NP_064550, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Immunofluorescence of monoclonal antibody to RAD18 (AT3545a) on HeLa cell . [antibody concentration 25 ug/ml]
Application
Western blot analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody (M01), clone 3H7 (Cat # AT3545a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
