Application
Western blot analysis of anti-Ran-GTPase Sumoylation site Pab (AP2503a) in HepG2 cell line lysate. Ran-GTPase Sumoylation site(arrow) was detected using the purified Pab.Application
Western blot analysis of Ran-GTPase (arrow) using rabbit polyclonal Ran-GTPase Antibody (Sumo) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ran-GTPase gene.| Product Name | RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:250~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1835, SD |
|---|---|
| Alternative Names | RANGAP1 KIAA1835 SD Ran GTPase-activating protein 1 |
| Molecular Weight(MW) | 63542 Da |
| Function | GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state |
| Tissue Specificity | Highly expressed in brain, thymus and testis. |
| Cellular Localization | Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Chromosome, centromere, kinetochore (By similarity). Cytoplasm, cytoskeleton, spindle pole (By similarity). Note=Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase Mitotic location also requires sumoylation |
Application
Western blot analysis of anti-Ran-GTPase Sumoylation site Pab (AP2503a) in HepG2 cell line lysate. Ran-GTPase Sumoylation site(arrow) was detected using the purified Pab.
Application
Western blot analysis of Ran-GTPase (arrow) using rabbit polyclonal Ran-GTPase Antibody (Sumo) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ran-GTPase gene.| Application Notes | WB:1:250~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
