Application
Western blot analysis on (A) HeLa, (B) MCF-7, (C) SH-SY5Y and (D) A549 cell lysates using anti-RanGAP1 RabMAb (Cat. #AJ1676a).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RanGAP1 RabMAb (Cat. #AJ1676a).Application
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-RanGAP1 RabMAb (Cat. #AJ1676a).Application
Immunofluorescent staining of HeLa cells using anti-RanGAP1 RabMAb (Cat. #AJ1676a).| Product Name | RanGAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3295 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:2000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1835, SD |
|---|---|
| Molecular Weight(MW) | 63542 Da |
| Function | GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state |
| Tissue Specificity | Highly expressed in brain, thymus and testis. |
| Cellular Localization | Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Chromosome, centromere, kinetochore (By similarity). Cytoplasm, cytoskeleton, spindle pole (By similarity). Note=Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase Mitotic location also requires sumoylation |
| Entrez Gene | 5905 |
|---|
Application
Western blot analysis on (A) HeLa, (B) MCF-7, (C) SH-SY5Y and (D) A549 cell lysates using anti-RanGAP1 RabMAb (Cat. #AJ1676a).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RanGAP1 RabMAb (Cat. #AJ1676a).
Application
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-RanGAP1 RabMAb (Cat. #AJ1676a).
Application
Immunofluorescent staining of HeLa cells using anti-RanGAP1 RabMAb (Cat. #AJ1676a).| Application Notes | WB:1:2000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
