Application
A. Western blot analysis on MCF-7 cell lysate using anti-RAR alpha RabMAb (Cat. #AJ1680a), dilution 1:1000.| Product Name | RAR alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RARA, NR1B1, Retinoic acid receptor alpha, Nuclear receptor subfamily 1 group B member 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1823Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NR1B1 |
|---|---|
| Alternative Names | RARA NR1B1 Retinoic acid receptor alpha Nuclear receptor subfamily 1 group B member 1 |
| Molecular Weight(MW) | 50771 Da |
| Function | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand- dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis |
| Tissue Specificity | A synthetic peptide corresponding to residues near N-terminal of human RAR alpha protein was used as an immunogen. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation |
| Entrez Gene | 5914 |
|---|
Application
A. Western blot analysis on MCF-7 cell lysate using anti-RAR alpha RabMAb (Cat. #AJ1680a), dilution 1:1000.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
