Application
Western blot analysis of Raptor-S863 in K562 cell line lysates (35ug/lane). Raptor (arrow) was detected using the purified Pab.Application
Raptor-S863 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Raptor-S863 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Raptor-S863 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Raptor-S863 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPTOR; KIAA1303; RAPTOR; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1303 RAPTOR |
|---|---|
| Alternative Names | RPTOR KIAA1303 RAPTOR Regulatory-associated protein of mTOR p150 target of rapamycin (TOR)-scaffold protein |
| Molecular Weight(MW) | 149038 Da |
| Function | Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates mTORC1 is activated in response to growth factors or amino acids Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation |
| Tissue Specificity | Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta |
| Cellular Localization | Cytoplasm. Lysosome. Note=Targeting to lysosomes depends on amino acid availability |
| Entrez Gene | 57521 |
|---|
Application
Western blot analysis of Raptor-S863 in K562 cell line lysates (35ug/lane). Raptor (arrow) was detected using the purified Pab.
Application
Raptor-S863 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Raptor-S863 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Raptor-S863 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
