Application
Western blot analysis of RAT Npm1 Antibody (Cat. #AM1837a) in rat heart tissue lysates (35ug/lane).NPM (arrow) was detected using the purified Mab.(1:2000)| Product Name | Rat Npm1 Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Npm1; Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3A9F1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Npm1 Nucleophosmin Nucleolar phosphoprotein B23 Nucleolar protein NO38 Numatrin |
|---|---|
| Molecular Weight(MW) | 32560 Da |
| Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules (By similarity). Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication (By similarity) |
| Tissue Specificity | This RAT NPM antibody is generated from mouse immunized with RAT NPM recombinant protein. |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm (By similarity). Cytoplasm, cytoskeleton, centrosome (By similarity) Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs Co-localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. NEK2 is required for its localization to the centrosome during mitosis (By similarity) |
| Entrez Gene | 25498 |
|---|
Application
Western blot analysis of RAT Npm1 Antibody (Cat. #AM1837a) in rat heart tissue lysates (35ug/lane).NPM (arrow) was detected using the purified Mab.(1:2000)| Application Notes | WB:1:500-2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Rat monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
